Currently Published Papers in Herbal Chemistry

Aonla and its processing – A review

G.H. Athawale and Dr. S.H. Akbari;June 2017 – August 2017; Sec. B; Vol.6, No.3, 62-73.DOI: 10.24214/IJGHC/HC/6/3/6273

Aonla is often known as Indian Gooseberry is considered to be an adaptogen which means that it has key compounds that normalize body functions and help the body to adapt to a variety of changes and stress conditions. Aonla is known for its medicinal and nutritional properties. This article will help different aonla processers as it consist details regarding different varieties of aonla and effect of different processing methods on the nutrient quality of aonla and different traditional products which are made using aonla.

In vitro inhibitory effect of Monodora myristica and Eucalyptus torelliana essential oils on the mycelial growth of Mycosphaerella fijiensis a pathogenic agent of the black leaf streak disease of banana and plantain

Camara Brahima1*, Amari Ler-N’ogn Dade Georges Elisee, Cherif Mamadou, Dick Emmanuel, Tuo Seydou, Kanko Coffi, Kone Daouda,IJGHC, June 2017 – August 2017; Sec. B; Vol.6, No.3, 51-61.,DOI: 10.24214/IJGHC/HC/6/3/5161

Banana and plantain whose fruits are a significant food source for the populations of Côte d’Ivoire, are sensitive to many diseases. Among them, the most significant due to the fungi is the black leaf streak disease caused by Mycosphaerella fijiensis which can involve more than 50 % of the losses of the production. The fight against this disease is primarily directed towards the use of synthetic fungicides. These substances, constitute a source of environmental pollution and foodstuffs rejection on the world market due to their residues. The biological fight using essential oils extracted from aromatic plants against this disease seems to be an alternative way. The present study was led in vitro to determine the antifongic activity of two essential oils extracted from Eucalyptus torelliana and Monodora myristica compared to a synthetic fungicide (spiroxamine), on the mycelial growth of Mycosphaerella fijiensis isolated from banana leaves in Côte d'Ivoire. Five concentrations of both essential oils and synthetic fungicide were added to the fungus culture medium (PDA). The mycelial growth was measured every 3 days during 21 days in the Petri dishes. The essential oil extracted from Monodora myristica presented a high fongitoxic effect at concentrations uper 7 000 ppm with a CI50 equal to 744.046 ppm. On the other hand the essential oil of Eucalyptus torelliana and synthetic fungicide had an increasing fongitoxic activity according to concentrations used. Their CI50 were respectively 3158.900 and 0.0145 ppm. Essential oils from the flora of Côte d’Ivoire can reduce or inhibit the mycelial growth of Mycosphaerella fijiensis and can constitute a means of effective biological fight against the black leaf streak disease.

Therapeutic effect of Portulace Olerecea leaves and seeds against hypercholesterolemia induced in rats

Afaf O. Ali and Nivin S.Nail

The objective of this study was to investigate the effect of dried leaves and seeds of purslane (Portulace Olerecea) on lipids levels in experimentally induced hypercholesterolemic rats. Thirty six male Albino rats were divided into 6 groups and reared for 1.5 months. The groups were: group (1) control, group (2) fed hypercholesterolemic diet (positive control), group (3) fed 10% grounded seed of Portulace Olerecea, group (4) fed  10% grounded leaves of Portulace Olerecea, group (5) hypercholesterolemic rats fed 10% grounded Portulace Olerecea seeds, group (6) hypercholesterolemic rats fed 10% grounded Portulace Olerecea leaves. At the end of experimental period, all rats were scarified and blood samples were taken for determination of liver function aspartate aminotransferase (AST), alanine aminotransferase (ALT), alkaline phosphatase (ALP) ,and lipid profile( triglyceride TG, cholesterol TC and high density lipoproteins HDL) determination. Also the fecal cholesterol and TG were determined. Liver and heart were taken for pathology analysis. The results showed that liver function activities (ALT, AST and ALP) were significantly decreased in the groups fed on 10% Portulace Olerecea seeds (gp.5) or leaves (gp.6) compared to the hyper-cholesterol group (gp.2). The levels of serum TG, TC were decreased in the group fed on 10% Portulace Olerecea seeds (gp.5) more than the group fed on 10% Portulace Olerecea leaves (gp.6) and both were better than hypercholesteralmic group (gp.2).While the HDL level was increased for both groups of Portulace Olerecea leaves or seeds compared to hypercholestolmic group which decreases in HDL. Fecal TC and TG levels were higher in groups fed 10% Portulace Olerecea leaves (gp.5) or seeds (gp.6) than hypercholesterolmic group (gp.2). Liver and heart histopathology showed more improvement in cell structure in groups fed Portulace Olerecea. The data also revealed that there is an improvement in lipid profile and lipid level discharged in the feces which suggested that Portulace Olerecea seeds reduces lipid in hypercholesteralmic rats and work as hepatoprotective by improving liver function more than leaves compared with high fat group.

Medium term in vitro conservation of two Argentinian oregano cultivars – preliminary results of the effect of mannitol and sorbitol as growth retardants

M. B. Marengo, P. Bima, M. S. Ojeda and L. E. Torres

The aim of this work was to develop an efficient protocol for medium term in vitro conservation of oregano. Two concentrations of mannitol and sorbitol (25 g.l-1 and 50 g.l-1) were tested to evaluate their effect as growth retardants over oregano plants belonging to Alpa Sumaj FCA – INTA and Aguanda FCA – INTA cultivars. Both cultivars are preserved in the in vitro collection of oregano germplasm of the Laboratorio de Biotecnologia Vegetal (Facultad de Ciencias Agropecuarias - Universidad Nacional de Córdoba). Every six weeks the length of major axis of plants (cm), fresh weight (g.plant-1) and dry weight (g.plant-1) were measured. At 24 weeks, plants were transferred to fresh MS control medium (active growing medium) to evaluate the plants response when mannitol and sorbitol were removed from the culture media. Regardless the cultivar, after 24 weeks of cultivation in slow growth conditions, best results were observed in plants growing in MS medium added with 25 g.l-1 of sorbitol; even though plants cultured on this medium were significantly smaller than those cultured on MS control medium, they showed a healthy appearance and when they were transferred to active growth medium its development was not significantly different from the control plants.

Evaluation of Skeletal Muscle Relaxant Activity of Ethanolic Extract of Lantana Camara Linn.

Lincy Joseph, Mathew George, Anmol Sharma, Naina Sara Sabu

The objective of present study was to evaluate the effect of the ethanolic extracts of lantana camara leaves in experimental animals for skeletal muscle relaxant activity. Ethanolic extract of lanata camara linn. Subjected to preliminary qualitative phtyochemical investigation and acute oral toxicity study for the test extract of the plant was carried out using OECD guidelines.  The skeletal muscle relaxant activity of the ethanolic extract of the drug (60mg/kg) was evaluated in rats using rota-rod apparatus. The diazepam was taken as the standard drug and results obtained after Ethanolic extract of lanata camara linn administration was compared with the standard drug and results obtained were significant up to 2 hr study as P‹ 0.01. The result suggest that the Ethanolic extract of lanata camara linn of herbal o